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试验研究了超低温贮存顽拗型(RecaIcitrant)种子茶籽的技术,结果表明,采用铝箔/薄膜复合袋密封包装,将含水量8％至13.83％的种子直接快速投入液氮,35℃温水解冻方式,能保持生活力,最适含水量为13.83％,经118天贮存后发芽率93.3％,且均成苗,液氮贮存后的最适发芽方法是在5％水分(W/W)沙床中于5±1℃预吸处理15天,然后移至25℃发芽,预处理后的种子,细胞膜修复能力增强,渗漏物减少,发芽率提高。
The results showed that seed with 8% -13.83% water content was quickly and directly poured into liquid nitrogen and thawed at 35 ℃ in warm water with aluminum foil / film composite bag. The results showed that the seed of RecaIcitrant seed tea was stored at low temperature. , Which can maintain the viability. The optimum water content was 13.83%. After 118 days storage, the germination rate was 93.3%, and all were seedlings. The optimal sprouting method after liquid nitrogen storage was in 5% water (W / W) Pre-adsorption treatment at 5 ± 1 ℃ for 15 days, then moved to 25 ℃ germinated, pre-treated seeds, cell membrane repair ability to enhance the leakage decreased, the germination rate increased.