论文部分内容阅读
目的探讨XPD的基因多态性与个体慢性苯中毒发病风险的关系。方法采用病例-对照设计,以152名苯中毒工人为病例组,152名接触苯而没有中毒表现的工人为对照组。应用聚合酶链反应-限制性片断长度多态性分析技术(PCR-RFLP)检测XPDc.199、XPDc.201、XPDc.312和XPDc.751位点的多态性。结果未检测到XPDc.199、XPDc.201位点的突变基因型;与携带XPDc.312Asp/Asp基因型的个体相比,调整性别、工龄和暴露强度后,携带XPDc.312Asp/Asn+Asn/Asn基因型的个体的慢性苯中毒的发病风险降低(ORadj=0.59,95%CI=0.35～0.99,χ2=3.99,P<0.05),在低强度苯接触组,该突变基因型的保护作用更为显著(ORadj=0.15,95%CI=0.04～0.51,χ2=8.93,P<0.01)。结论XPDAsp312Asn基因多态可能与个体慢性苯中毒发病风险的改变有关。
Objective To investigate the relationship between polymorphism of XPD and the risk of developing chronic benzene poisoning. Methods A case-control study was designed. A total of 152 workers with benzene poisoning were selected as the case group and 152 workers who were exposed to benzene without poisoning were selected as the control group. The polymorphisms of XPDc.199, XPDc.201, XPDc.312 and XPDc.751 were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results The genotypes of XPDc.199 and XPDc.201 were not detected. Compared with individuals carrying the XPDc.312Asp / Asp genotype, XPDc.312Asp / Asn + Asn / Asn genotype had a lower risk of chronic benzene poisoning (ORadj = 0.59, 95% CI = 0.35-0.99, χ2 = 3.99, P <0.05). In the low-intensity benzene exposure group, the genotype was more protective (ORadj = 0.15, 95% CI = 0.04-0.51, χ2 = 8.93, P <0.01). Conclusion XPDAsp312Asn polymorphism may be related to the risk of chronic benzene poisoning.