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针对细胞表面抗原的单克隆抗体(Mab)的生产,需要一种筛选系统,以便从众多杂交克隆中选出所需特异性的克隆株.以往所用的方法包括补体依赖的细胞毒试验、间接细胞膜荧光试验(IMF)等.但这些方法费时、操作繁琐,因此不能用于大量样本的检验.本文报道将细胞固定在微孔盘上,再进行固相放射免疫测定或ELISA等.基本原理是使用植物凝集素加上戊二醛共同交联.具体作法是:将平底ELISA盘的每一
The production of monoclonal antibodies against cell surface antigens (Mabs) requires a screening system to select clones of the desired specificity from a large number of hybrid clones. Previously used methods include complement-dependent cytotoxicity assays, indirect cell membranes Fluorescence assay (IMF), etc. However, these methods are time-consuming and cumbersome to operate and therefore can not be used for testing a large number of samples.In this paper, the cells are fixed on a microporous plate and subjected to solid-phase radioimmunoassay or ELISA, etc. The basic principle is to use Plant lectin plus glutaraldehyde cross-linked by the specific practice is: each flat ELISA plate