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为制备抗马来丝虫感染期幼虫表膜抗原(S)、排泄分泌抗原(ES_m),以及马颈盘尾丝虫微丝蚴排泄分泌抗原(ES_c)的单克隆抗体,作者介绍了一种脾内注射活虫体的快捷免疫方法。脾内抗原注射后3～4天进行细胞融合。较之传统方法省时,免疫所用虫量亦少。 BALB/c ByJ小鼠(6～8周♀),用乙醚麻醉,于腹部脾区作一长2cm切口,暴露脾尖。为使抗原在脾内均匀分布,25号针纵向刺入脾一定深度,边退针边注入含活虫体的无血清培养液50μl,3～4天后取脾。
In order to prepare monoclonal antibodies against larvae surface antigens (S), excretory-secretory antigens (ES_m), and excretion-secreting antigens (ES_c) of filarial microfilariae on silkworms, the authors present a Spleen injection of live worms fast immunization methods. Cells were fused 3 to 4 days after spleen antigen injection. Compared with the traditional method of saving time, the amount of insects used for immunization is also less. BALB / c ByJ mice (6-8 weeks old) were anesthetized with ether and a 2 cm long incision was made in the spleen area of the abdomen to expose the spleen. In order to make the antigen evenly distributed in the spleen, the 25th needle penetrates into the spleen longitudinally to a certain depth, inject 50μl of serum-free culture solution containing live worms into the spores and withdraw the spleen 3 to 4 days later.