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从50对AFLP引物中筛选出9对多态性高、扩增稳定、重复性好的引物,对敦化一化型、洮南二化型和公主岭二化型3个地理种群共24个个体进行遗传多样性分析,研究不同化性亚洲玉米螟间的遗传关系。结果表明,9对引物共扩增出1 127条清晰可辨的DNA条带,其中多态性条带1 029条,平均多态性比例为91.30%;亚洲玉米螟不同种群个体间遗传相似性指数分布在0.430 7～0.667 5之间;同一种群内个体间的遗传相似性指数敦化一化型为0.439 9～0.524 0,洮南二化型为0.487 8～0.667 5,公主岭二化型为0.459 7～0.563 9,遗传相似性较低,表明亚洲玉米螟遗传多样性十分丰富。聚类分析结果表明,供试的24个亚洲玉米螟聚成两大类,敦化一化型聚为一类,洮南二化型和公主岭二化型聚为一类。
Ninety pairs of AFLP primers were screened out from 50 pairs of primer pairs with high polymorphism, stable amplification and good reproducibility. A total of 24 individuals from three geographic populations of Dunhua-1, Tienan-2 and Gongzhuling-2 were selected Analysis of genetic diversity and the genetic relationship among different sexes of Asian corn borer. The results showed that a total of 1 127 clear DNA bands were amplified by 9 pairs of primers, of which 1029 were polymorphic bands with the average percentage of 91.30%. Genetic similarity among different populations of Asian corn borer The distribution of the index was between 0.430 7 and 0.667 5. Among the individuals in the same population, the genetic similarity index of Dunhua normalized type was 0.439 9-0.524 0, that of Taonan type was 0.487 8-0.667 5, that of Gongzhuling second type was 0.459 7 ~ 0.563 9, genetic similarity is low, indicating that the Asian corn borer genetic diversity is very rich. The results of cluster analysis showed that the 24 Asian corn borers were clustered into two major groups, Dunhua-type was clustered into one type, and Taonan-II type and Gongzhuling-type were clustered together.