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目的:梓醇对脑缺血大鼠的促血管新生效应。方法:雄性SD大鼠24只,随机分为假手术组、模型组、生理盐水组、梓醇治疗组(剂量为5 mg.kg-1),每组6只。采用开颅电凝右侧大脑中动脉的方法制备局灶脑缺血模型。造模后6 h,经腹腔注射首次给予梓醇治疗,每天1次,连续7 d。术后15 d断头取脑,观察脑表面血管分布特点;制备脑片并采用荧光双标记染色,检测微血管密度和新生微血管数目,评价梓醇治疗脑缺血后血管新生作用;采用透射电镜,观察梓醇对脑微血管内皮细胞超微结构的影响。结果:脑缺血后15 d,模型组可见缺血区脑组织液化、坏死,脑表面血管分枝数目稀少,排列紊乱;梓醇治疗组病灶基本愈合,脑表面血管分枝数目比模型组明显增加。荧光双标记结果显示,梓醇治疗组病灶区均有显著的血管新生,血管新生数目和微血管密度均较模型组显著增加(P<0.05)。内皮超微结构显示,梓醇治疗组内皮水肿较模型组轻,基膜基本正常,内皮细胞可见较多的线粒体,已接近正常状态。结论:梓醇可促进脑缺血大鼠缺血周围区皮质血管新生,同时改善脑血管内皮细胞肿胀。
OBJECTIVE: Effect of catalpol on angiogenesis in rats with cerebral ischemia. Methods: Twenty-four male Sprague-Dawley rats were randomly divided into sham operation group, model group, saline group and catalpol treatment group (5 mg.kg-1) with 6 rats in each group. The model of focal cerebral ischemia was made by craniotomy electrocoagulation on the right middle cerebral artery. 6 h after modeling, the first intraperitoneal injection of catalpol treatment, 1 day, for 7 days. Fifteen days after operation, the brain was decapitated to observe the distribution of blood vessels on the surface of the brain. Brain slices were prepared and stained with fluorescent double labeling to detect the density of microvessels and the number of newborn capillaries. The effects of catalpol on angiogenesis were evaluated by transmission electron microscopy, To observe the effect of catalpol on the ultrastructure of cerebral microvascular endothelial cells. Results: At 15 days after cerebral ischemia, cerebral ischemia and liquefaction occurred in the model group, and the number of blood vessel branches on the surface of the brain was sparse and arranged in disorder. The lesions in the catalpol group basically healed, and the numbers of blood vessel branches on the brain surface were significantly higher than those in the model group increase. Fluorescence double labeling results showed that there were significant angiogenesis in the tumor area of catalpol treatment group, the number of angiogenesis and microvessel density were significantly increased (P <0.05) compared with model group. The ultrastructure of endothelial cells showed that the endothelial edema of catalpol-treated group was lighter than that of the model group, the basilar membrane was normal and the endothelial cells showed more mitochondria, which was close to the normal state. Conclusion Catalpol can promote cortical angiogenesis in the ischemic region and improve the swelling of cerebral vascular endothelial cells in rats with cerebral ischemia.