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目的 探讨儿童急性淋巴细胞白血病(ALL)nm23-H_1基因的表达水平及其与免疫分型的关系.方法 应用半定量逆转录-聚合酶链反应(RT-PCR)的方法检测儿童ALL初治、缓解、难治、复发患儿及正常对照儿童nm23-H_1基因的表达水平,观察nm23-H_1基因与免疫表型的关系.结果 ①初治患儿nm23-H_1表达水平明显高于正常对照组(P<0.01)和完全缓解组(P<0.01);完全缓解组nm23-H_1表达水平与对照组差异无显著性(P0.05),复发组nm23-H_1表达水平高于正常对照组(P<0.01)和完全缓解组(P<0.01),与初治差异无显著性(P0.05);难治组均为高表达.②T-ALL的mn23-H_1阳性率高于B-ALL(P<0.05),且T-ALL的nm23-H_1表达水平高于B-ALL(P<0.05).结论 nm23-H_1的表达水平随儿童ALL的病情发展而变化,在初治、复发及难治ALL具有高表达,nm23-H_1高表达者预后差,易复发;nm23-H_1在T-ALL具有高表达,可能是T-ALL的缓解率低、预后差的原因之一.“,”Objective To study the expression of nm23-H_1 gene in children with acute lymphoblastic leukemia (ALL) and the relationship between nm23-H, expression and immunophenotype. Methods nm23-H_1 expression was measured by semiquantitative RT-PCR in children with ALL (newly diagnosed, n =40; remission, n = 32; relapse, n = 16; refractory, n =3). Twenty normal children served as the control group. The relationship between nm23-H_1 expression and immunophenotype was evaluated. Results The expression of nm23-H_1 in the newly diagnosed ALL group was significantly higher than that in the control (P<0. 01 ) and the remission groups (P<0.01 ). There was no difference in the nm23-H, expression between the remission and the control groups. The expression of nm23-H_1 in the relapse group was significantly higher than that in the control (P < 0. 01) and the remission groups (P < 0.01), and similar to that in the newly diagnosed ALL group. The three children with refractory ALL had higher nm23-H, expression. Both the positive rate and expression of nm23-H, in children with T-lineage ALL were higher than in children with B-lineage ALL (P < 0.05 ). Conclusions The expression level of nm23-H_1 varies with the stages of ALL. Newly diagnosed, relapsed and refractory ALL children have higher nm23-H_1 expression. High nm23-H_1 expression may be associated with a poor prognosis and relapse. A higher expression of nm23-H_1 in children with T-ALL may be contributed to a low remission rate and a poor prognosis.