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目的探讨三氧化二砷(As2O3)对肝细胞癌(HCC)组织ezrin基因表达及血清甲胎蛋白(AFP)水平的影响。方法 24例可切除HCC患者,其中男性20例,女性4例;年龄42～74岁,平均年龄为58.6岁。术前接受As2O3治疗14d(10mg/d,静脉滴注)。取治疗前的病灶活组织检查标本和治疗后手术切除标本,采用免疫组织化学方法均行ezrin表达检测,同时比较治疗前、后血清AFP水平的变化,并分析血清AFP水平与ezrin基因表达的相关性。结果治疗前ezrin表达强阳性、弱阳性和阴性例数分别为6例、7例和11例,而As2O3治疗后分别为2例、5例和17例,两组比较差异有统计学意义(χ2=5.619,P<0.05)。治疗前血清AFP为325.5ng/L,治疗后AFP为278.6ng/L,前后比较差异有统计学意义(Z=-2.360,P<0.05)。结论 As2O3可明显下调HCC肿瘤细胞ezrin表达,有抑制HCC细胞生长、抑制复发转移的作用。
Objective To investigate the effect of arsenic trioxide (As2O3) on ezrin gene expression and serum AFP level in hepatocellular carcinoma (HCC). Methods Twenty-four patients with HCC were resected, including 20 males and 4 females, aged from 42 to 74 years with a mean age of 58.6 years. As2O3 treatment for 14 days (10mg / d, intravenous drip) before surgery. The specimens of preoperative lesions and biopsies were collected. The ezrin expression was detected by immunohistochemistry. The changes of serum AFP levels before and after treatment were compared. The level of serum AFP was correlated with ezrin gene expression Sex. Results There were 6 cases, 7 cases and 11 cases of ezrin expression positive, weak positive and negative cases before treatment, respectively, and 2 cases, 5 cases and 17 cases after As2O3 treatment, the difference was statistically significant (χ2 = 5.619, P <0.05). Before treatment, serum AFP was 325.5ng / L, AFP was 278.6ng / L after treatment, the difference was statistically significant before and after (Z = -2.360, P <0.05). Conclusion As2O3 can down-regulate ezrin expression in HCC tumor cells, inhibit HCC cell growth and inhibit the recurrence and metastasis.