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目的明确高脂饮食后改为普通饮食对大鼠非酒精性脂肪性肝病(NAFLD)的防治效果。方法 23只SD大鼠随机分为正常对照组(NC,n=8)、高脂模型组(HF,n=7)、高脂后改正常饲料组(HF+CD,n=8),16周后处死大鼠。检测大鼠体重、肝重、血生化、血脂肪细胞因子及肝脏TG水平;检测肝脏脂质合成及炎症因子mRNA及肝脏病理。结果与HF组比较,HF+CD组肝脏指数[(3.10±0.58)%vs(2.43±0.17)%]、ALT[(210.66±39.19)vs(175.00±18.94)U/L]、血清TGI-(0.71±0.08)vs(0.51±-0.14)mmol/L]、肝脏TG[(7.94-±-1.38)vs(6.09±1.62)μmol/L]及肝脏NF-κB mRNA[(2.28±0.24)vs(1.60±0.25)]均下降(P<0.05或P<0.01),其他指标及肝脏病理改变不明显。结论高脂饮食后改普通饮食对高脂NAFLD模型大鼠有治疗作用,但更积极的药物、限制热卡等治疗可能更有效。
Objective To clarify the prevention and treatment of non-alcoholic fatty liver disease (NAFLD) in rats after high-fat diet changed to general diet. Methods Twenty - three SD rats were randomly divided into normal control group (NC, n = 8), high fat model group (HF, n = 7), high fat diet group (HF + CD, n = 8) Rats were sacrificed weeks later. The body weight, liver weight, blood biochemistry, blood adipocytokines and hepatic TG levels in rats were measured. Lipid synthesis, inflammatory cytokines and liver pathology were detected. Results The liver index of HF + CD group was significantly higher than that of HF group (3.10 ± 0.58% vs 2.43 ± 0.17%, ALT 210.66 ± 39.19 vs 175.00 ± 18.94 U / L, 0.71 ± 0.08 vs 0.51 ± -0.14 mmol / L], hepatic TG [(7.94 ± ± 1.38) vs (6.09 ± 1.62) μmol / L] and hepatic NF- 1.60 ± 0.25)] were decreased (P <0.05 or P <0.01), other indicators and liver pathological changes were not obvious. CONCLUSIONS: Diet modification after high-fat diet may have a therapeutic effect on rats with high-fat NAFLD. However, it may be more effective to treat the patients with more active drugs and restrict heat card.