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本文报告应用肝癌新鲜组织细胞悬液制备抗人HCC的Mab,采用组织学免疫酶标记定位技术进行抗体的检测,获得一株具有肝癌相关的单克隆抗体Ab20,用此抗体在福尔马林固定石蜡切片肝癌组织进行组织学定位,35例中29例阳性,阳性率82.9％,与增生胆小管上皮,正常胃粘膜及胰腺腺泡上皮,肠癌、胃癌组织存在不同程度的交叉反应,而正常肝、胎儿肝及胚胎肝组织中无Ab20相应抗原的表达,对Ab20相应抗原的特性及Ab 20的应用价值有待于进一步的研究。
This paper reports the preparation of anti-human HCC Mab using fresh tissue cell suspension of HCC and detecting the antibody by using the histochemical immunoenzyme marker localization technology to obtain a monoclonal antibody Ab20 with liver cancer, which was fixed in formalin Paraffin sections of liver cancer tissue histological localization, 35 cases of 29 cases of positive, the positive rate of 82.9%, and proliferative bile duct epithelium, normal gastric mucosa and pancreatic acinar epithelium, intestinal cancer, gastric cancer tissue varying degrees of cross-reaction, and There is no Ab20 antigen expression in normal liver, fetal liver and embryonic liver tissue. The characteristics of Ab20 antigen and the value of Ab 20 remain to be further studied.