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目的 检测乙型肝炎患者血清白细胞介素 1 0 (IL 1 0 )并探讨其临床意义。方法 用双抗体夹心ELISA法检测 2 0 0 0 - 0 2～ 2 0 0 1 - 0 5广东医学院附属医院 49例住院的乙型肝炎患者和 40位健康人血清IL 1 0质量浓度 ,同时检测肝功能和乙肝病毒标记物 ,分析不同类型肝炎患者血清IL 1 0水平差异及其慢性肝炎组中IL 1 0与HBeAg的关系。结果 各型肝炎患者血清IL 1 0质量浓度与正常对照比较 ,急性肝炎组 (AHB)、慢性肝炎组 (CHB)、重型肝炎组 (SHB)血清IL 1 0质量浓度分别为 ( 79 0 9± 2 1 5 )ng/L ,( 88 2 0± 2 2 1 )ng/L ,( 97 44± 3 1 6)ng/L ,显著高于正常对照组 ( 67 34± 1 2 5 )ng/L ,P值均小于 0 0 5。组间比较 :CHB组高于AHB组 ,P值 <0 0 5 ,低于SHB组 ,P <0 0 0 1。CHB组中HBeAg阳性组明显高于HBeAg阴性组 (P <0 0 5 )。结论 提示IL 1 0参与乙型肝炎的病理反应过程 ,其升高可能与HBV持续感染有关 ,也可能做为反映乙型肝炎病情轻重程度的指标。
Objective To detect serum interleukin 10 (IL 1 0) in patients with hepatitis B and to explore its clinical significance. Methods Serum IL 1 0 concentrations of 49 hospitalized patients with hepatitis B and 40 healthy subjects from 2000 to 2000-2001-01 in Guangdong Medical College Hospital were detected by double antibody sandwich ELISA. Liver function and hepatitis B virus markers, analysis of different types of hepatitis patients serum IL 1 0 levels and its chronic hepatitis group IL 10 and HBeAg relationship. Results Compared with the normal control group, the serum concentrations of IL-10 in all kinds of hepatitis patients were (79 0 9 ± 2) in acute hepatitis group (CHB) and severe hepatitis group (SHB) L, (88 2 0 ± 2 2 1) ng / L and (97 44 ± 3 1 6) ng / L respectively, which was significantly higher than that of the control group (67 34 ± 125 2 ng / L, P values are less than 0 0 5. Comparison between groups: CHB group was higher than AHB group, P value was less than 0 0 5, lower than SHB group, P 0 001. The HBeAg positive group in CHB group was significantly higher than that in HBeAg negative group (P <0.05). The conclusion suggests that IL 1 0 is involved in the pathological reaction of hepatitis B, which may be related to persistent HBV infection or may be used as an index to reflect the severity of hepatitis B.