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我们在1990～1991年作了两年的诱导培养基和分化培养基的对比试验。结果表明,N_6基本培养基附加NAA的N_6-1诱导培养基和附加NAA和2,4-D的N_6-2诱导培养基的愈伤组织诱导率分别为30.3％和25.3％,它比附加2,4-D的常用的N_6。诱导培养基(愈伤组织诱导率为20.5％)高出47.8％和23.4％。NAA和2,4-D混用的N_6-2去分化培养基所诱导出的愈伤组织的器官分化率为最高,其绿苗分化率为57.4％,它比常用的N_5培养基(绿苗分化率为44.8％)高出28.0％。器官分化率最低的是单用NAA的N_6-1培养基,其绿苗分化率为36.4％,比N_6培养基低18.7％。附加NAA的N_6-1和N_6-2培养基的出愈速度和器官分化速度明显快于附加2,4-D的N_5培养基。N_6-1培养基和N_6-2培养基花药接种后18天就能看到愈伤组织,出愈速度要比N_5培养基提早3～5天。在N_6培养基上的形成的愈伤组织转移后10天只有部分绿苗出现,但N_4-1和N_6-2培养基上形成的愈伤组织转移后10天,可分化出1/3的绿苗,甚至转移后3天可以看到不少绿点分化。分化培养基来看,MS培养基的植物体分化效率明显高于N_6培养基,其绿苗率提高到2.5倍。
We conducted a two-year comparative study of induction and differentiation media between 1990 and 1991. The results showed that the callus induction rates of N_6-1 medium supplemented with NAA and N_6-2 medium supplemented with NAA and 2,4-D were respectively 30.3% and 25.3% , 4-D commonly used N_6. Induction medium (callus induction rate was 20.5%) was 47.8% and 23.4% higher. The differentiation rate of callus induced by N_6-2 dedifferentiation medium with NAA and 2,4-D was the highest, the rate of green plantlet differentiation was 57.4%, which was higher than that of common N_5 medium The rate was 44.8%) 28.0% higher. The lowest organ differentiation rate was N_6-1 medium with NAA alone. The differentiation rate of green shoots was 36.4%, which was 18.7% lower than that of N_6 medium. The rate of emergence and organ differentiation of NAA-supplemented N_6-1 and N_6-2 medium was significantly faster than that of N_5 medium supplemented with 2,4-D. Callus could be seen 18 days after inoculation of N_6-1 medium and N_6-2 medium anther, the recovery rate was 3 to 5 days earlier than N_5 medium. Only some of the green shoots appeared on the 10th day after the formation of callus on N_6 medium, but 10 days after the callus formed on N_4-1 and N_6-2 medium could be differentiated by 1/3 Miao, or even 3 days after the transfer can see a lot of green point differentiation. Differentiation medium point of view, MS medium plant differentiation efficiency was significantly higher than N_6 medium, the green shoot rate increased to 2.5 times.