论文部分内容阅读
[目的]通过监测,了解广东省人群及宿主动物中钩体病菌群的变化,为预防钩体病的爆发流行,合理有效地制定预防措施。[方法]采集相关人群及宿主动物血清用显微镜凝集试验(MAT)进行抗体测定;采集相关人群全血、宿主动物脏器做钩端螺旋体分离培养及分群鉴定。[结果]健康人血清、疑似病人血清、宿主动物血清平均抗体阳性率分别为33.90%、4.59%、36.69%;在疑似病人血中分离培养出秋季热群钩体1株;在鼠肾中分离培养出21株钩端螺旋体,其中爪哇群14株、秋季热群4株、黄疸出血群2株和澳洲群1株;在猪肾、蛙肾中均未分离到钩端螺旋体。[结论]广东省人群及宿主动物钩体隐性感染水平较高,人群中血清抗体阳性的菌型以黄疸出血群为多,而宿主动物以爪哇群为多。鼠类动物脏器分离培养及血清抗体阳性率较高,显示老鼠仍然是传播钩体的主要传染源之一,提示我们要警惕由于菌群的更迭而引起钩体病的爆发流行。
[Objective] To understand the changes of Leptospira gonorrhoeae population in Guangdong province and host animals through monitoring. In order to prevent the outbreak of leptospirosis, it is reasonable and effective to make preventive measures. [Method] The serums of the relevant population and host animal were collected for the determination of antibody by using the microscope agglutination test (MAT). The whole blood of the relevant population was collected and the organism of the host animal was isolated and cultured for Leptospira interrogans. [Results] The average antibody positive rates of serum from healthy people, suspected patients and serum from host animals were 33.90%, 4.59% and 36.69% respectively. One isolate was isolated and cultured in the blood of suspected patients, Twenty-one strains of Leptospira were cultured, among which 14 strains were Java, 4 in autumn, 2 in jaundice and 1 in Australia. [Conclusion] The prevalence of latent infection in livers of host and host animals in Guangdong Province is relatively high. The majority of seropositive antibodies in populations are mostly hemorrhagic jaundice, while host animals are mostly Java. Isolation and culture of murine organ and serum antibody positive rate is high, indicating that the mice are still the main source of transmission Leptospira, suggesting that we must guard against changes in the flora caused by leptospirosis outbreak.