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绿脓杆菌的感染较多,尤其是灼伤创面的感染更为普遍,临床上要求作出快速正确之诊断,目前的常规检查,还不能满足要求。特别当遇到不典型菌落,如肉眼看不到产生之色素,或色素产生不明显时,就需要进一步种半固体琼脂观察;但对“不产生”色素(肉眼看不到色素产生)的绿脓杆菌的诊断就发生困难,此时就需以醣发酵试验,或氧化酶试验,进行鉴定,但需时较久。我们在工作的过程中,发现绿脓杆菌产生之色素,在紫外线照射下,发出鲜绿色之萤光。依据此现象,我们试验了60余株绿脓杆菌,其结果完全符合诊断。
Pseudomonas aeruginosa infection more, especially burns wound infection is more common, clinically required to make a quick and correct diagnosis, the current routine examination, still can not meet the requirements. Especially when it comes to atypical colonies, such as the naked eye can not see the pigment produced, or the pigment is not obvious, you need to further observation of semi-solid agar; but not to produce "pigment (pigment production can not see the naked eye) green Pseudomonas occurred in the diagnosis of difficulties, this time on the need to sugar fermentation test, or oxidase test, identification, but takes longer. During our work, we found that the pigment produced by Pseudomonas aeruginosa emits a bright green fluorescence under UV irradiation. Based on this phenomenon, we tested more than 60 strains of Pseudomonas aeruginosa, the results in full compliance with the diagnosis.