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在建立了耐顺铂 （ＣＤＤＰ） 的卵巢癌细胞的基础上， 探讨了敏感及耐药的卵巢癌细胞株胞膜上的ＡＴＰ酶（ＡＴＰａｓｅ） 活性。结果表明： 两株肿瘤细胞膜上的Ｎａ＋ 、Ｋ＋ －ＡＴＰａｓｅ 和Ｃａ２＋ 、Ｍｇ２＋ －ＡＴＰａｓｅ 活性均被抑制后， 耐药细胞膜上的ＡＴＰａｓｅ活性明显高于敏感细胞（Ｐ＜ ０．０５）， 而且该ＡＴＰａｓｅ活性可在维拉帕米（ＶＰＭ） 作用下进一步增高，与未加ＶＰＭ 的耐药细胞相比差异有显著性（Ｐ＜ ０．０５）。提示肿瘤细胞耐药的产生可能与细胞膜上的ＡＴＰａｓｅ活性改变有关。
Based on the establishment of cisplatin-resistant (CDDP) ovarian cancer cells, the ATPase (ATPase) activity on the membrane of sensitive and resistant ovarian cancer cell lines was investigated. The results showed that the activities of Na+, K+-ATPase and Ca2+ and Mg2+-ATPase were inhibited on the membranes of the two tumors, and the ATPase activity on the membrane of drug-resistant cells was significantly higher than that of the sensitive cells (P<0.05). It can be further increased by verapamil (VPM), and the difference is significant (P < 0.05) compared with those without VPM. It suggests that the development of drug resistance in tumor cells may be related to changes in ATPase activity on the cell membrane.