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【目的】研究不同余氯浓度和暴露时间对细菌的去除效果,分析不同余氯条件对细胞ATP的影响。【方法】以河水中微生物群落为试验对象,利用流式细胞术(Flow cytometry,FCM)评估不同余氯浓度和暴露时间的灭活效果,检测不同余氯浓度时细胞内(外)ATP的变化情况。【结果】不同余氯浓度和暴露时间对细菌的去除效果产生不同的结果。在余氯浓度<2 mg/L情况下,延长氯暴露时间可以增加细菌的去除效果,在余氯浓度≥2 mg/L条件下,较短氯暴露时间就可以灭活90%细菌。高核苷酸细菌(HNA)和低核苷酸细菌(LNA)表现出不同氯耐受能力,且HNA细菌相比LNA细菌较容易受到氯的损伤。细胞内ATP随余氯浓度增加而减少,在高浓度余氯条件下(≥2 mg/L)细胞外ATP才会增加。【结论】微生物活性随着余氯作用的增加而降低,FCM法和ATP检测法可以用于评估加氯消毒对微生物稳定性的影响。
【Objective】 The purpose of this study was to investigate the effect of different residual chlorine concentration and exposure time on the removal of bacteria, and to analyze the effect of different residual chlorine on cellular ATP. 【Method】 The microbial communities in river water were used as experimental subjects. Flow cytometry (FCM) was used to evaluate the inactivation effect of different residual chlorine concentration and exposure time. The changes of intracellular (extracellular) ATP concentration Happening. 【Result】 Different concentrations of residual chlorine and exposure time had different effects on the removal of bacteria. When the residual chlorine concentration is lower than 2 mg / L, prolonging the exposure time can increase the bacterial removal efficiency. When the residual chlorine exposure time is shorter than 2 mg / L, 90% bacteria can be inactivated. High nucleotide (HNA) and low nucleotide (LNA) bacteria show different tolerance to chlorine, and HNA bacteria are more susceptible to chlorine damage than LNA bacteria. The intracellular ATP decreased with the increase of residual chlorine concentration, and the extracellular ATP increased under the condition of high residual chlorine (≥2 mg / L). 【Conclusion】 The activity of microorganism decreased with the increase of residual chlorine. FCM and ATP assay could be used to evaluate the influence of chlorination on microbial stability.