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[目的]建立鸡肉中己烯雌酚残留量的高效液相色谱测定方法。[方法]用乙醇萃取鸡肉中的己烯雌酚残留,采用固相萃取柱(SPE),甲醇一水(10+90)2ml淋洗,甲醇2ml洗脱,收集洗脱液浓缩蒸干,再用甲醇溶解残渣,用紫外检测器高效液相色谱仪,在波长280nm处检测,并使用外标法定量。[结果]平均回收率可达到72.2%以上,最低检测浓度10μg/kg。[结论]建立的高效液相色谱测定鸡肉中己烯雌酚残留量的方法与其他方法相比具有操作简便、快速、精密度高、回收率高、重现性较好,检测限低等优点。
[Objective] To establish a method for the determination of diethylstilbestrol in chicken by high performance liquid chromatography. [Method] The diethylstilbestrol residue in chicken meat was extracted by ethanol and eluted by solid phase extraction column (SPE), 2ml methanol (10 + 90), 2ml methanol. The eluate was concentrated and evaporated to dryness, then dissolved in methanol The residue was detected by UV detector HPLC at a wavelength of 280 nm and quantified using an external standard. [Result] The average recovery rate could reach 72.2% and the lowest detection concentration was 10μg / kg. [Conclusion] The established HPLC method for the determination of diethylstilbestrol in chicken meat compared with other methods has the advantages of simple, rapid, high precision, high recovery, good reproducibility and low detection limit.