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Based on the c DNA sequences from hyper variable(HV) regions of identified 52 S-alleles in Oriental pear cultivars, S-RNase c DNA probes were designed, and a c DNA microarray for S-RNase detections was established. Each microarray contained 240 sites from 55 c DNA probes, including all specific c DNA sequences from the HV regions of the S-alleles. Using the c DNA of pistils of tested pear cultivars as template and Cy3 fluorescently labeling primers by PCR amplification, microarray hybridization detected the S-genotype of each pear cultivar. The genotypes inferred from the c DNA microarray hybridization signals of pear cultivars such as ‘Lijiang Huangsuanli’, ‘Xiuyu’, ‘Midu Yuli’, ‘Baimianli’, and ‘Deshengxiang’ were similar to the known genotypes of all tested cultivars. The S-RNase c DNA microarrays and the oligonucleotide gene chips were then used to conduct parallel testing of 24 P. pyrifolia cultivars with unknown S-genotypes. In conclusion, the construction of c DNA microarrays has further improved the pear S-RNase detection platform.
Based on the c DNA sequences from hyper variable (HV) regions of identified 52 S-alleles in Oriental pear cultivars, S-RNase c DNA probes were designed, and ac DNA microarray for S-RNase detections was established. Each microarray contained 240 sites From the c DNA sequences, including all specific c DNA sequences from the HV regions of the S-alleles. Using the c DNA of pistils of tested pear cultivars as template and Cy3 fluorescently labeled primers by PCR amplification, microarray hybridization detected the S- of each pear cultivar. The genotypes inferred from the c DNA microarray hybridization signals of pear cultivars such as ’Lijiang Huangsuanli’, ’Xiuyu’, ’Midu Yuli’, ’Baimianli’, and ’Deshengxiang’ were similar to the known genotypes of all tested cultivars. The S-RNase c DNA microarrays and the oligonucleotide gene chips were then used to conduct parallel parallel testing of 24 P. pyrifolia cultivars with unknown S-genotypes. In conclusion, the construction of c DNA micrograms oarrays has further improved the pear S-RNase detection platform.