论文部分内容阅读
采用热水法提取药材枸杞子中的枸杞多糖。本研究通过MTT实验发现3.125～200mg/L质量浓度范围内的枸杞多糖能显著抑制宫颈癌Hela细胞的增殖;采用荧光显微镜、透射电镜和激光共聚焦扫描显微镜观察发现经枸杞多糖处理过的Hela细胞呈现出典型的凋亡特征;原位末端脱氧核苷酸转移酶标记法进一步证实经枸杞多糖处理过的Hela细胞呈现凋亡特征。结果提示枸杞多糖是一种潜在的抗癌复合物,其关键作用机制是诱导癌细胞凋亡。
Lycium barbarum polysaccharide extracted by hot water method. In this study, it was found by MTT assay that LBP in the concentration range of 3.125 ~ 200mg / L could significantly inhibit the proliferation of cervical cancer Hela cells. Fluorescence microscopy, transmission electron microscopy and confocal laser scanning microscopy showed that LBP treated Hela cells Showing the typical characteristics of apoptosis; in situ terminal deoxynucleotidyl transferase labeling method further confirmed that LBP treated Hela cells showed apoptosis characteristics. The results suggest that lycium barbarum polysaccharide is a potential anti-cancer complex, the key mechanism of which is to induce cancer cell apoptosis.