论文部分内容阅读
目的:探索大黄抑制鼠疫耶尔森菌作用的分子机制。方法:液体稀释法测定大黄水提物对鼠疫菌生长抑制作用的最小浓度(MIC)。应用鼠疫耶尔森菌全基因组芯片研究大黄作用鼠疫菌的表达谱。选择10倍MIC做为大黄抑制鼠疫菌作用浓度,作用时间分别选择30,60min。提取并纯化鼠疫耶尔森菌总RNA;逆转录合成cDNA;用Cy3,Cy5染料标记后,与鼠疫耶尔森菌全基因组芯片杂交;通过芯片扫描仪获得表达谱分析结果。应用SAM软件对结果进行分析;应用RT-PCR对芯片结果进行验证。结果:得到了大黄不同作用时间鼠疫菌的表达谱。获得了大黄不同作用时间鼠疫菌的相同差异表达基因。结论:大黄抑制鼠疫菌的主要分子机制在于影响细菌核糖体蛋白质合成相关基因与细胞膜相关基因的表达。
Objective: To explore the molecular mechanism of rhubarb inhibiting the action of Yersinia pestis. Methods: Liquid dilution method was used to determine the minimum concentration (MIC) of the inhibitory effect of rhubarb aqueous extract on the growth of Y. pestis. A Yersinia pestis whole-genome microarray was used to study the expression profile of Rye bacillus against Y. pestis. 10 times MIC was selected as the inhibitory concentration of ryegrass for rhubarb, and the time of action was 30 and 60 min. The total RNA of Yersinia pestis was extracted and purified; cDNA was synthesized by reverse transcription; after labeling with Cy3, Cy5 dyes, it was hybridized with Yersinia pestis whole genome chip; expression profile analysis results were obtained by a chip scanner. The results were analyzed using SAM software; the results of the chip were verified by RT-PCR. RESULTS: The expression profile of Y. pestis at different time was obtained. The same differentially expressed genes of Yersinia pestis at different time of rhubarb were obtained. Conclusion: The main molecular mechanism of rhubarb inhibiting Y. pestis is to affect the expression of genes related to bacterial ribosome protein synthesis and cell membrane related genes.