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目的对悬铃木属花粉变应原进行初步纯化并对其主要成分进行免疫学活性分析。方法悬铃木属花粉变应原粗制浸液经过凝胶过滤层析,收集主要蛋白质,SDS-PAGE检测各段蛋白质分子量,并用免疫印记法分析7例悬铃木属花粉过敏的支气管哮喘患者血清。结果悬铃木属花粉变应原粗制液经层析柱洗脱得两个洗脱峰,第一峰及第二峰升段富含蛋白,而第二峰降段蛋白含量甚微,收集各段进行SDS-PAGE,出现6条蛋白区带,分子量分别为7、50、35、39、22和16kd。第一峰主要含22￣71kd蛋白质,峰2升段以14￣16kd之蛋白质为主。对7例花粉过敏的支气管哮喘患者血清免疫印记分析可见4条sIgG反应带,蛋白分子量为50、39、22和16kd,病人血清结合百分比分别为100%、28.57%、57.14%和14.29%。结论悬铃木属花粉变应原含有6种主要蛋白成分,第一峰的50、39和22kd的蛋白质为主要致敏组分,第二峰升段的16kd蛋白质为次要致原。
Objective To preliminarily purify the pollen allergen of Platycladus and analyze the immunological activity of its main components. Methods The crude extracts of Platanus pollen allergens were subjected to gel filtration chromatography and the main proteins were collected. The molecular weight of each segment was determined by SDS-PAGE, and the sera of 7 patients with asthma allergies were analyzed by immunoblot. Results The crude solution of Platanus pollen allergen was eluted with two elution peaks on the chromatographic column. The first peak and the second peak were rich in protein, while the second peak was very low in protein content. Each segment was collected. After SDS-PAGE, there were six protein bands with molecular weights of 7, 50, 35, 39, 22, and 16 kd. The first peak contains 22 to 71 kd of protein, and the peak of 2 liters is mainly 14 to 16 kd. The results of serum immunoblot analysis of 7 asthmatic patients with pollen allergy showed that there were 4 sIgG reaction bands with protein molecular weights of 50, 39, 22, and 16 kd, and the patient serum binding percentages were 100%, 28.57%, 57.14%, and 14.29%, respectively. Conclusion The P. edulis pollen allergens contained six major protein components. The 50, 39 and 22 kd proteins of the first peak were the main sensitized components, and the 16 kd protein of the second peak rose to be the minor.