论文部分内容阅读
目的应用简便的方法对棘阿米巴角膜炎进行诊断，并对棘阿米巴原虫进行鉴定。方法角膜刮片或培养的原虫材料经１０％氢氧化钾液湿封片镜检；角膜材料经原虫培养；应用倒置显微镜对棘阿米巴属进行观察和鉴定；角膜病理切片经ＨＥ和ＰＡＳ染色。结果１０％ＫＯＨ湿封片镜检，５例角膜刮片中有２例及培养阳性的原虫材料均呈现棘阿米巴原虫的包囊。同一病例，４例中有３例培养出棘阿米巴原虫。在倒置显微镜下可见棘阿米巴原虫的包囊和滋养体以及滋养体上的棘状伪足。病理切片经ＨＥ和ＰＡＳ染色均显示原虫的包囊。结论１０％ＫＯＨ湿封片可对棘阿米巴角膜炎作初步诊断，最后诊断需经原虫培养获得，应用倒置显微镜可对棘阿米巴原虫进行观察和鉴定。角膜病理切片上看到原虫可进一步印证原虫培养的结果。
Objective To diagnose Acanthamoeba keratitis by simple method and to identify Acanthamoeba sp. Methods The corneal scraps or cultured protozoa were microscopically examined with 10% potassium hydroxide solution. The corneal material was cultured in protozoal. The acanthamoeba was observed and identified by inverted microscope. The corneal biopsies were stained with HE and PAS . Results 10% KOH wet packing microscopy, 5 cases of corneal scraping in 2 cases and culture-positive protozoa materials were present Acanthamoeba cysts. In the same case, 3 out of 4 cases grew Acanthamoeba protozoa. In the inverted microscope can be seen Acanthamoeba cysts and trophozoites and trophozoites spines pseudopodia. Pathological sections by HE and PAS staining showed protozoa cysts. Conclusions 10% KOH wet packs can be used for the preliminary diagnosis of Acanthamoeba keratitis. The final diagnosis should be obtained by protozoal culture. The invertebrate microscope can be used to observe and identify the protozoan Acanthamoeba. Protozoa seen on corneal biopsy can further confirm the protozoa culture results.