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目的:研究壳聚糖絮凝沉降法对蒽醌类成分的影响。方法:选择大黄为研究对象,采用壳聚糖沉降法处理大黄水提液,以大黄酸、大黄素、芦荟大黄素、大黄酚、大黄素甲醚质量分数和浸膏率为指标,选取药液质量浓度、壳聚糖加入量、药液pH为考察因素,通过正交试验优选壳聚糖絮凝沉降工艺;并与乙醇沉淀法比较。结果:优选的壳聚糖絮凝沉降工艺为药液质量浓度0.1 g.mL-1,壳聚糖加入量10%,药液pH 6。壳聚糖沉淀法和乙醇沉淀法均能较好地使水提液沉淀,但两者均会造成蒽醌类成分的损失,前者在降低浸膏率、保留有效成分方面优于后者。2种沉淀法纯化后,药液中蒽醌类成分与未纯化药液比较无显著性差异。结论:壳聚糖絮凝沉降法对蒽醌类成分影响较乙醇沉淀法小,可用于含蒽醌类成分中药的沉淀,但是否适合于大生产有待进一步研究。
Objective: To study the effect of chitosan flocculation and sedimentation on anthraquinone components. Methods: The rhubarb was selected as the research object. The chitosan sedimentation method was used to treat Rhubarb water extract. Rhein, emodin, chrysophanol, chrysophanol, physcion, Mass concentration, the amount of chitosan added and the pH value of the liquid as the factors for investigation. Chitosan flocculation settling process was optimized by orthogonal test; and compared with ethanol precipitation method. Results: The optimal chitosan flocculation and settling process was that the mass concentration of the drug solution was 0.1 g.mL-1, the amount of chitosan added was 10%, and the pH value of the liquid was 6. Chitosan precipitation method and ethanol precipitation method can make the water extract precipitation, but both will cause the loss of anthraquinones components, the former in reducing the extract rate, retaining the active ingredient is better than the latter. After the two precipitation methods were purified, there was no significant difference between anthraquinones and unpurified liquid in the solution. Conclusion: The flocculation and settling method of chitosan has a smaller effect on the anthraquinones than the ethanol precipitation method, which can be used for the precipitation of traditional Chinese medicine containing anthraquinones. However, whether the method is suitable for large-scale production remains to be further studied.