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用植物血凝素微分沉淀法从体外培养物中分离出W_1株裂殖子。在3小时内的分离过程中,将这些裂殖子悬浮在磷酸缓冲液中,在液氮中冷冻,于-80C下保存;另一方法是将悬浮在10%正常恒河猴血清水溶液中的裂殖子冻干。冷冻和冻干的疫苗在使用前保存12周。接种注射之前,疫苗用福氏完全佐剂(FCA)乳化,而后给猴肌内注射2次,间隔3～4周一次,每只猴接种裂殖子的总量为4×10~9个。所有接种疫苗的猴和正常对照猴都用诺氏疟原虫W_1和W_3变异株的未成熟裂殖体血液静脉注射进行攻击。结果正常猴发生了迅速上升的原虫血症,并在攻击后的第6和
W_1 merozoites were isolated from in vitro cultures by phytohemagglutinin differential precipitation. During 3 hours of isolation, these merozoites were suspended in phosphate buffer, frozen in liquid nitrogen and stored at -80 ° C. Alternatively, the merozoites were suspended in 10% normal rhesus monkey serum in water Mealogen lyophilized. Frozen and lyophilized vaccines are kept for 12 weeks prior to use. Prior to vaccination, the vaccine was emulsified with Freund’s Complete Adjuvant (FCA) and then injected intramuscularly twice a week for 3 to 4 weeks. The total amount of merozoites in each monkey was 4 × 10-9. All vaccinated monkeys and normal control monkeys were challenged with immature splenic blood by intravenous injection of P. nidulans W_1 and W_3 mutants. Results Normal monkeys developed a rapid increase in parasitemia and were at 6th and 6th post-challenge