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为了研究阿片类物质对LHRH和SRIF释放的作用,将成年雄性大鼠的内侧基底下丘脑(MBH)切片在37℃条件下,用氧合Hepes缓冲液Locke液灌流一小时。加入浓度为2×10~(-5)M的杆菌肽以防止神经肽的酶降解。K~+脉冲液流(Pulse)6分钟可明显刺激这两个肽的释放。阿片类(β-内啡肽10~(-7)M;D—丙_2—甲硫—脑啡肽10~(-7)M;亮—脑啡肽10~(-7);吗啡10~(-6)M)不改变LHRH和SRIF的自发性释放,但却能明显地抑制K~+诱导的神经
To investigate the effect of opioids on LHRH and SRIF release, medial basal hypothalamic (MBH) sections of adult male rats were perfused with Locke’s solution of oxygenated Hepes buffer for one hour at 37 ° C. Bacitracin at a concentration of 2 x 10 ~ (-5) M was added to prevent enzymatic degradation of neuropeptides. K ~ + pulse for 6 minutes significantly stimulated the release of both peptides. Opioids (β-endorphin 10 ~ (-7) M; D-propane-2-methylthio-enkephalin 10 ~ (-7) M; ~ (-6) M) did not change the spontaneous release of LHRH and SRIF, but significantly inhibited the K ~ + -induced neural