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本文研究了几种病因不同的男性不育症病人精浆及血浆中的β-ED(Beta-endorphin)水平及其与血中雄性激素的关系,以便使人们更好地理解内源性鸦片物质对男性生育力的影响和它们对男性不育症是否有诊断价值。实验对象为年龄在23～34岁的男性50例。其中正常对照13例,精索静脉曲张14例,阻塞性无精症5例,非阻塞性无精症6例,原因不明的精子活力不足症12例。每例实验对象均作了精液常规检查,包括精子密度、精子运动、活力及精子形态。不育症的诊断按惯例进行。将液化后的精液和经EDTA处理后的血液分别经4℃低温离心后,立即取上清液(即精浆和血浆)贮存于-70℃低温下待用。
This article studies the levels of β-endorphin (β-ED) and its association with androgen in blood in seminal plasma and plasma of several male infertility patients in order to provide a better understanding of endogenous opioid The impact on male fertility and their diagnostic value for male infertility. The subjects were 50 males and females aged 23 to 34 years old. Among them, 13 cases were normal control, 14 cases were varicocele, 5 cases were obstructive azoospermia, 6 cases were non-obstructive azoospermia, 12 cases were unexplained sperm motility insufficiency. Sperm routine tests were performed on each subject, including sperm density, sperm motility, vitality and sperm morphology. Infertility diagnosis according to practice. The liquefied semen and EDTA-treated blood were centrifuged at 4 ℃, immediately after the supernatant (ie, seminal plasma and plasma) stored at -70 ℃ low temperature stand-by.