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AIM:To investigate the effect of Lianshu preparation on lipopolysaccharide (LPS)-induced diarrhea in rats.METHODS: A diarrhea model was established in Sprague Dawley rats via injection of 1 mL of 30 mg/kg LPS. A total of 40 rats were randomly divided into normal group, LPS group, LPS + Lianshu group, LPS + berberine group (n=10 in each group). Their intestinal mucosal barrier and frequency of diarrhea were observed. Levels of glucose, serum Na+, K+, Cl- and hematocrit, plasma nitrogen monoxide (NO), diamine oxidase (DAO), and D (-)-lactate were measured. The number of IgA+ plasma cells in small intestine was detected and SIgA levels in the intestinal fluid were measured. The antipyretic activity of Lianshu preparation in rats was evaluated using Brewer’s yeast-induced pyrexia (10 mL/kg of 20% aqueous suspension). Acetaminophen (250 mg/kg, intragastric administration, bid) was used as a standard drug for comparison. Temperature was recorded 1 h before and 6 h after Brewer’s yeast injection. Finally, small intestinal transmission in mice treated with Lianshu was detected after intraperitoneal injection of methyl prostigmin (2 mg/kg). Atropine (10 g/kg) was used as a control. The ink content in intestine was determined and the total length of intestine was measured.RESULTS: The frequency of diarrhea was higher in LPS group than in LPS + Lianshu group and LPS + berberine group (36.70±5.23 vs 28.50 ±4.06 and 32.70±9.30 respectively, P<0.01), and lower in LPS + Lianshu group than in LPS + berberine group (P=0.03). The levels of Na+, glucose, Cl-, K+ were significantly lower in LPS + Lianshu group than in LPS + berberine group (140.35±3.19 mmol/L vs 131.99±4.86 mmol/L, 8.49 ±1.84 mmol/L vs 6.54±2.30 mmol/L, 106.29±4.41 mmol/L vs 102.5±1.39 mmol/L, 5.08±0.66 mmol/L vs 4.32±0.62 mmol/L respectively, p<0.05). The level of hematocrit was lower in LPS + Lianshu group than in LPS + berberine group (0.50% ± 0.07% vs 0.59% ± 0.10% respectively, P<0.05). The plasma levels of NO, DAO and D (-)-lactate were higher in LPS group than in normal group (79.74±7.39 μmol/L vs 24.94±3.38 μmol/L, 2.48±0.42 μ/mL vs 0.82±0.33 μ/mL, 5.63±0.85 μg/mL vs 2.01±0.32 μg/mL respectively, P<0.01), and lower in LPS + Lianshu group than in LPS+berberine group (48.59±4.70 μmol/L vs 51.56±8.38 μmol/L, 1.43±0.53 μmol/mL vs 1.81±0.42 μmol/mL, 4.00±0.54 μg/mL vs 4.88±0.77 μg/mL respectively, P<0.05). The morphology of the intestinal mucosa showed destroyed villi in LPS group and atrophied intestinal mucosa in other groups. The pathological intestinal mucosal changes were less in LPS + Lianshu group than in LPS group. The number of IgA+ plasma cells and amount of SIgA were higher in LPS + Lianshu group than in LPS group (1.16±0.19/μm2 vs 1.09±0.28/μm2, P=0.026; 0.59±0.12 mg/L vs 0.15±0.19 mg/L respectively, P=0.000). Lianshu had counteractive effects on yeast-induced pyrexia and enterokinesia in rats.CONCLUSION: Lianshu preparation has therapeutic effects on LPS-induced diarrhea and enterokinesia in rats.
AIM: To investigate the effect of Lianshu preparation on lipopolysaccharide (LPS) -induced diarrhea in rats. METHODS: A diarrhea model was established in Sprague Dawley rats via injection of 1 mL of 30 mg / kg LPS. A total of 40 rats were randomly selected Levels of glucose, serum Na +, K +, Cl- and hematocrit (n = 10 in each group); LPS + The number of IgA + plasma cells in small intestine was detected and SIgA levels in the intestinal fluid were measured. The antipyretic activity of Lianshu Preparation in rats was evaluated using Brewer’s yeast-induced pyrexia (10 mL / kg of 20% aqueous suspension). Acetaminophen (250 mg / kg, intragastric administration, bid) was used as a standard drug for comparison. and 6 h after Brewer’s yeast inject ion. Finally, small intestinal transmission in mice treated with Lianshu was detected after intraperitoneal injection of methyl prostigmin (2 mg / kg). Atropine (10 g / kg) was used as a control. The ink content in intestine was determined and the total length of intestine was measured.RESULTS: The frequency of diarrhea was higher in LPS group than in LPS + Lianshu group and LPS + berberine group (36.70 ± 5.23 vs 28.50 ± 4.06 and 32.70 ± 9.30 respectively, P <0.01), and lower in in LPS + Lianshu group than in LPS + berberine group (P = 0.03). The levels of Na +, glucose, Cl-, K + were significantly lower in LPS + Lianshu group than in LPS + berberine group (140.35 ± 3.19 mmol / L vs 131.99 ± 4.86 mmol / L, 8.49 ± 1.84 mmol / L vs 6.54 ± 2.30 mmol / L, 106.29 ± 4.41 mmol / L vs 102.5 ± 1.39 mmol / L, 5.08 ± 0.66 mmol / L vs 4.32 ± 0.62 mmol / L respectively, p <0.05). The level of hematocrit was lower in LPS + Lianshu group than in LPS + berberine group (0.50% ± 0.07% vs 0.59% ± 0.10% respectively, P <0.05) els of NO, DAO and D (-) - lactate were higher in LPS group than in normal group (79.74 ± 7.39 μmol / L vs 24.94 ± 3.38 μmol / L, 2.48 ± 0.42 μ / mL vs 0.82 ± 0.33 μ / / mL vs 2.01 ± 0.32 μg / mL respectively, P <0.01), and lower in LPS + Lianshu group than in LPS + berberine group (48.59 ± 4.70 μmol / L vs 51.56 ± 8.38 μmol / L, 1.43 ± 0.53 μmol / vs 1.81 ± 0.42 μmol / mL, 4.00 ± 0.54 μg / mL vs 4.88 ± 0.77 μg / mL respectively, P <0.05). The morphology of the intestinal mucosa showed destroyed villi in LPS group and atrophied intestinal mucosa in other groups. The pathological The number of IgA + plasma cells and amount of SIgA were higher in LPS + Lianshu group than in LPS group (1.16 ± 0.19 / μm2 vs 1.09 ± 0.28 / μm2, P = 0.026; 0.59 ± 0.12 mg / L vs. 0.15 ± 0.19 mg / L respectively, P = 0.000). Lianshu had countertractive effects on yeast-induced pyrexia and enterokinesia in rats. -induced diarrhea and enterokinesia in rats.