【摘 要】
：
Sophora moorcroftiana (S.moorcroftiana) is an endemic leguminous dwarf shrub in Tibet,China.Decoctions of the seeds have been used in Chinese folk medicine for dephlogistication,detoxication,and infectious diseases.The present study aimed to investigate t
【机 构】
：
Department of Immunology, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, Chin
论文部分内容阅读
Sophora moorcroftiana (S.moorcroftiana) is an endemic leguminous dwarf shrub in Tibet,China.Decoctions of the seeds have been used in Chinese folk medicine for dephlogistication,detoxication,and infectious diseases.The present study aimed to investigate the constituent and biological effects of polysaccharides from S.moorcroftiana seeds in Caenorhabditis elegans (C.elegans).Polysaccharides from S.moorcroftiana seeds (SMpol) were extracted with 60％ ethanol and constituent was analyzed by GC-MS.SMpol was composed of glucose,galactose and inositol in the molar ratio of 35.7 ∶ 1.3∶17.0.Synchronized worms were treated with SMpol and then lifespan,motility,reproduction,stress resistance and antimicrobial activity were examined.Compared with the control group,the lifespan was increased to the average of 27.3 days and the number of laying eggs showed a 1.3-fold increase in nematodes treated with SMpol (4 mg·mL-1).In SMpol (4 mg.mL-1) treated worms,there was a 1.1-fold increase in 24-h survival of acute heat stress and a 1.6-fold increase in 2-h survival of oxidative stress The colonization of the bacteria in the SMpol treated nematode was significantly lower than that of the untreated group by 68.3％.In vivo studies showed SMpol significantly extended the life span,improved reproduction,increased stress resistance and antimicrobial capacity of C.elegans.In conclusion,those results indicated that the polysaccharides from S.moorcrofiiana seeds were involved in a variety of biological activities leading to its modulatory effects on C.elegans which may be developed as a natural supplement agent.
其他文献
Astragali Radix,the root of Astragalus membranaceus (Fisch.) Bge.var.mongholicus (Bge.) Hsiao or Astragalus membranaceus (Fisch.) Bge.,is widely used as a tonic decoction pieces in the clinic of traditional Chinese medicine (TCM).Astragali Radix has vario
Sophorae Flavescentis Radix (Sophora flavescens Ait.,SFR) and Sophorae Tonkinensis Radix et Rhizoma (S.tonkinensis Gapnep.,STR) are two commonly used traditional Chinese medicines from Sophora (Leguminosae) plants,which are believed to possess similar bio
Gnaphalium affine D. Don, a medicinal and edible plant, has been used to treat gout in traditional Chinese medicine and popularly consumed in China for a long time. A detailed phytochemical investigation on the aerial part of G. affine led to the isolatio
Catalpol, a major bioactive component from Rehmannia glutinosa, which has been used to treat diabetes. The present study was designed to elucidate the anti-diabetic effect and mechanism of action for catalpol in db/db mice. The db/db mice were randomly di
Analysis errors can occur in the desorbing process of ginkgo diterpene lactone meglumine injection (GDMI) by a conventional analysis method, due to several factors, such as easily crystallized samples, solvent volatility, time-consuming sample pre-process
The present study was designed to evaluate protective activity of an ethanol extract of the stems ofSchisandra chinensis (SCE) and explore its possible molecular mechanisms on acetaminophen (APAP) induced hepatotoxicity in a mouse model.The results of HPL
Multi-components in herbal formulae exert holistic effects in synergistic or additive manners.However,appropriate strategies and supportive evidences are still lacking to uncover the synergistic or additive combinations.The present investigation aimed at
Four new 3,4-seco-labdane diterpenoids,nudiflopenes J-M,were isolated from the leaves of Callicarpa nudiflora along with six known compounds.The structures of these diterpenoids were determined by comprehensive spectroscopic analysis.All the isolated comp
This study developed a population pharmacokinetic model for sodium tanshinone ⅡA sulfonate (STS) in healthy volunteers and coronary heart disease (CHD) patients in order to identify significant covariates for the pharmacokinetics of STS.Blood samples were
The present study was designed to develop a sensitive and selective high performance liquid chromatography-tandem mass spectrometric method for the determination of Camellianin A in HepG2 cells.The extraction of Camellianin A was achieved using 15％ trichl