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在有机化合物制备性分离时常采用洗脱柱层析法,但此法存在着费时,费溶剂等缺点,由此发展了玻璃干柱层析法,但采用玻璃柱对者,荧光性物质又存在着不易观察和切割等缺点。当上样量大,柱径增加时色带不整齐更难切割,鉴于上述缺点,又有用塑料柱者,虽解决了观察和切割等问题,但不易填装、塑料薄膜对溶剂的选性强,且不可反复利用,故其应用受到了很大限制。
Eluting column chromatography is often used for the preparation of organic compounds. However, this method has the disadvantages of time-consuming and solvent-consuming. Therefore, glass dry column chromatography has been developed. However, the presence of fluorescent substances in glass columns is also present. Difficult to observe and cut. When the sample volume is large and the ribbon diameter is increased, it is more difficult to cut the ribbon when it is not neat. In view of the above shortcomings, the plastic column is also used. Although the problems such as observation and cutting are solved, it is difficult to fill, and the plastic film is highly selective for the solvent. , and can not be repeatedly used, so its application has been very limited.