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目的:探讨力竭运动后不同时相心肌核呼吸因子1(NRF-1)的变化特点。方法:90只健康成年雄性SD大鼠,分为一次力竭游泳运动组、2周反复力竭游泳运动组及安静对照组,分别于力竭运动后即刻、6、12及24小时取材,应用免疫荧光组化技术和图像分析方法研究大鼠心肌NRF-1蛋白含量的变化。结果:一次力竭游泳运动后即刻组大鼠心脏各部位(左心室、室间隔、右心室)中NRF-1蛋白含量均显著低于对照组(P<0.05),同时即刻组左心室NRF-1蛋白含量显著低于其他各组(P<0.05),即刻组和12小时组室间隔NRF-1蛋白表达均达到最低值,而24小时组右心室NRF-1蛋白含量显著低于对照组(P<0.01);反复力竭运动后6小时组和24小时组,大鼠心脏各部位蛋白含量显著低于对照组(P<0.05),力竭后24小时组与对照组相比具有显著性差异(P<0.05)。结论:一次力竭运动和反复力竭运动可导致大鼠NRF-1蛋白表达明显降低,力竭运动后12小时室间隔NRF-1蛋白含量显著低于其他各部位,这可能是运动性心肌微损伤和心律失常发生的重要机制之一。
Objective: To investigate the changes of myocardial nuclear factor of 1 (NRF-1) at different phases after exhaustive exercise. Methods: Ninety healthy adult male Sprague-Dawley rats were divided into two groups: one exhaustive swimming group, two exhaustive swimming group and quiescent control group. The rats were drawn at 6, 12 and 24 hours after exhaustive exercise respectively. Immunofluorescence staining and image analysis were used to investigate the changes of NRF-1 protein in rat myocardium. Results: The content of NRF-1 protein in the left ventricle, ventricular septum and right ventricle of rats immediately after exhaustive swimming was significantly lower than that of the control group (P <0.05). At the same time, the left ventricular NRF- (P <0.05). The expression of NRF-1 protein in ventricular septum was the lowest in the immediate group and the 12-hour group, while the content of NRF-1 protein in the right ventricle in the 24-hour group was significantly lower than that in the control group (P <0.01). The protein content in the heart of rats in 6 hours and 24 hours after exhaustive exercise was significantly lower than that in control group (P <0.05), and there was significant difference between 24 hours after exhaustive exercise and control group Difference (P <0.05). Conclusion: After exhaustive exercise and repeated exhaustive exercise, the expression of NRF-1 protein in rats decreased significantly. The content of NRF-1 protein in ventricular septum at 12 hours after exhaustive exercise was significantly lower than that in other sites, One of the important mechanisms of injury and arrhythmia.