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目的分析人类肠道病毒71型(EV71)基因特征及流行病学信息。方法用生物信息学软件对来自13个国家、中国的17省、直辖市不同时间内分离的270株EV71病毒VP1区基因序列进行分析,计算不同毒株的核苷酸差异率以及基因型/亚型内部、之间的同源性,构建系统发生树,分析流行病学信息。结果 270株病毒可分为3个基因型(A、B、C型),B型和C型又各可分为5个亚型;其中A型只有3株,B型和C型占了98.9%。不同毒株的核苷酸差异率为7.1%~25.9%,不同基因型之间的核苷酸差异率为23.0%~25.9%。同时从发生树中发现一些区别于B型和C型的“孤儿株”。结论同一地区可以出现某种基因型/亚型流行很长时间,同一时间内可以出现不同的基因型/亚型在某地区流行,具有明显的时间和地域流行特点。“孤儿株”的发现为EV71的不断进化以及具有基因多态性提供了证据。
Objective To analyze the characteristics and epidemiological information of human enterovirus 71 (EV71) gene. Methods The bioinformatics software was used to analyze the sequence of VP1 region of 270 strains of EV71 virus isolated from different provinces in 17 provinces and municipalities of China in 13 countries. The nucleotide variation rate and genotype / subtype of different strains were calculated. Internal and homology, construct phylogenetic tree and analyze epidemiological information. Results 270 viruses were divided into 3 genotypes (A, B and C), and B and C genotypes were divided into 5 subtypes. There were only 3 A genotypes and 98.9 genotype B and C genotypes %. The nucleotide variation rates of different strains ranged from 7.1% to 25.9%, and the nucleotide differences among different genotypes ranged from 23.0% to 25.9%. At the same time, we found some “orphan strains” that are different from B and C from the occurring tree. Conclusion In the same area, some genotypes / subtypes may occur for a long time, and different genotypes / subtypes may appear in one area at the same time with obvious temporal and geographical epidemic characteristics. The discovery of orphan strains provided evidence of the evolution of EV71 and its genetic polymorphism.