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为了检定人α干扰素制品中活性分子,本文作者观察比较了人α干扰素的部分提纯制品[PPHuIFN-α]和高度纯化的均一性制品[HHuIFN-α]在抑制肿瘤细胞DNA合成和促进NK细胞活性方面的效应。在DNA合成抑制试验中,作者采用微量法检测不同纯度干扰素制品对肿瘤细胞DNA合成的影响。作者使用的淋巴母细胞干扰素(Namalva细胞)[IFN-α(Ly)],部分提纯制品的比活性为1.25×10~5u/mg蛋白,均一性制品的比活性为2.5×10~3u/mg蛋白,分子量18,500;使用了不同的肿瘤细胞系包括人肿瘤细胞K_(562)、U_(937)(人巨噬细胞样细
To test for active molecules in human interferon alpha preparations, the authors observed the effects of partially purified human IFNα product [PPHuIFN-α] and highly purified homogenous product [HHuIFN-α] on the inhibition of DNA synthesis and NK Effect on cell activity. In the DNA synthesis inhibition test, the authors used the microdialysis method to detect the effect of different purity interferon products on the DNA synthesis of tumor cells. The specific activity of the partially purified product was 1.25 × 10 ~ 5u / mg protein, and the specific activity of the homogenous product was 2.5 × 10 ~ 3u / mg. The lymphocyte interferon (Namalva cells) [IFN- mg protein, molecular weight 18,500; different tumor cell lines including human tumor cells K_ (562), U_ (937) (human macrophage-like