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室内条件下用西芹腐根乙醇、丙酮和蒸馏水浸提液,连续5代处理黄瓜枯萎病菌,测定处理后黄瓜枯萎病菌分泌的镰刀菌酸量和细胞壁降解酶活性及其与致病力的相关关系,以揭示西芹腐根浸提液连续多代处理黄瓜枯萎病菌的致弱机制。结果表明,各处理的致病力随接种代数的增加逐渐减小,镰刀菌酸质量浓度和果胶酶活性随培养代数的增加逐代减少,纤维素酶活性随培养代数的增加无明显的变化规律,β-葡萄糖苷酶活性随培养代数的增加逐代升高,但均表现为处理低于对照。相关分析表明,致病力与菌体分泌的镰刀菌酸质量浓度和果胶酶活性均表现为显著或极显著的正相关关系,与纤维素酶活性相关性不显著;与培养第3~5代β-葡萄糖苷酶活性表现为显著或极显著的正相关关系。
Under the indoor conditions, the ethanolic, acetone and distilled water extract of celery root were used to treat the cucumber Fusarium oxysporum f. Sp. For 5 successive generations, and the acidity and the activity of cell wall-degrading enzyme secreted by Fusarium oxysporum f. In order to reveal the mechanism of weakening of Fusarium oxysporum f. The results showed that the pathogenicity of each treatment decreased gradually with the increase of inoculation algebra, the concentration of Fusaric acid and pectinase activity decreased with the increase of culture algebra, and the activity of cellulase did not change obviously with the increase of culture algebra The activity of β-glucosidase increased with the increase of culture algebra, but both of them showed lower than control. Correlation analysis showed that pathogenicity and fungal secretion of Fusaric acid concentration and pectinase activity showed a significant or very significant positive correlation between the activity and cellulase activity was not significant; and culture of 3 to 5 The generation of β-glucosidase activity showed a significant or very significant positive correlation.