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成年个体的B淋巴细胞主要来源于骨髓,有报道表明IL-4在B细胞成熟和分化过程中具有支持怍用,但机制尚不清楚,为此作者就IL-4对前B 细胞的直接作用进行了研究。鉴于骨髓细胞的异质性,首先纯化其中的前B 细胞:取2～3月龄(C3H/eb C57BL/6)F1小鼠的骨髓细胞,根据前B 细胞表面有B220分子而无sIg 特点,即B220~+sIg~-细胞,以相应的荧光抗体进行标记,FACS 加以分离。结果所得细胞纯度>95%,sIg~+细胞<0.2%。该细胞做为前B 细胞用于下述实验。首先发现在没有支持细胞或因子时,一部分前B 细胞主要是小前B 细胞能够分化为B细胞。当加入一定数目的非前B 细胞(B220-
B-lymphocytes in adult individuals are mainly derived from bone marrow. It has been reported that IL-4 has a supporting role in B-cell maturation and differentiation. However, the mechanism remains unclear. To this end, the authors have investigated the direct effects of IL-4 on pre-B cells Were studied. In view of the heterogeneity of myeloid cells, first of all, pre-B cells were purified: Bone marrow cells of F1-3 mice aged 2-3 months (C3H / eb C57BL / 6) were stained with B220 molecule without sIg on the surface of pre- Namely B220 ~ + sIg ~ - cells, labeled with the corresponding fluorescent antibody, FACS to be separated. Results The resulting cell purity> 95%, sIg ~ + cells <0.2%. The cells were used as pre-B cells for the following experiments. It was first discovered that in the absence of supporting cells or factors, a subset of pre-B cells, primarily small pre-B cells, can differentiate into B cells. When adding a number of non-pre-B cells (B220-