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目的建立锯缘青蟹(Scylla serrata)原肌球蛋白(tropomyosin,TM)的食物过敏性小鼠模型,并对其致敏性进行评价。方法锯缘青蟹TM以灌胃方式刺激小鼠使其致敏,并对小鼠粪便组胺、血清IgE以及脾脏细胞IL-4、IL-13、IFN-λ等淋巴因子的水平进行检测。结果 TM组小鼠均出现了不同程度的过敏症状,其血清IgE的含量是阴性对照组(生理盐水)小鼠的2倍;TM组实验小鼠IL-4、IL-13、IFN-λ的水平分别为(17.508±2.189)pg/ml、(3.150±0.434)pg/ml、(15.056±1.974)pg/ml,粪便中组胺含量为6 244.651 nmol/l,与阴性对照组小鼠相比均有显著差异(P<0.05)。结论锯缘青蟹TM以灌胃方式刺激小鼠,TM组小鼠出现了不同程度的过敏症状,其粪便组胺、血清IgE以及脾脏细胞IL-4、IL-13、IFN-λ等淋巴因子的水平与阴性对照组小鼠相比均有显著差异(P<0.05),建立的TM过敏动物模型可望为蟹类过敏的诊断和免疫治疗提供技术依据。
Objective To establish a food allergic mouse model of Scylla serrata tropomyosin (TM) and evaluate its sensitization. Methods Mud crab TM was stimulated by gavage to sensitize mice. The levels of histamine, serum IgE and IL-4, IL-13 and IFN-λ in spleen cells of mice were measured. Results The mice in TM group developed allergic symptoms in varying degrees and had twice as many serum IgE levels as those in the negative control (saline) mice. In the TM group, the levels of IL-4, IL-13 and IFN-λ (17.508 ± 2.189) pg / ml, (3.150 ± 0.434) pg / ml and (15.056 ± 1.974) pg / ml, and the histamine content in the feces was 6 244.651 nmol / l, respectively. Compared with the negative control mice There were significant differences (P <0.05). CONCLUSION: TM mice could stimulate mice by intragastric administration. TM mice showed different degrees of allergic symptoms. The histamine, serum IgE and spleen lymphocytes such as IL-4, IL-13 and IFN-λ (P <0.05). The animal model of TM allergy is expected to provide a technical basis for the diagnosis and immunotherapy of crab allergy.