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目的研究大鼠肠道水解酶对淫羊藿所含黄酮成分淫羊藿苷、朝藿定A、朝藿定B、朝藿定C在肠道处置的影响。方法应用大鼠在体肠灌流模型,用高效液相法测定药物浓度,比较淫羊藿黄酮苷在加入肠道乳糖酶根皮苷水解酶(LPH酶)抑制剂葡糖酸内酯(gluconolactone)前后表观渗透系数的变化。结果加入抑制剂后,4个淫羊藿黄酮苷在大鼠十二指肠、空肠、回肠、结肠的表观渗透系数都有不同程度的下降,其中淫羊藿苷在4个肠段的水解抑制率分别为:62%,50%,40%,46%;朝藿定A分别为:55%,26%,21%,14%;朝藿定B分别为:42%,40%,74%,22%;朝藿定C分别为:42%,40%,52%,35%。结论LPH酶抑制剂葡糖酸内酯能不同程度地抑制淫羊藿苷、朝藿定A、朝藿定B、朝藿定C在大鼠肠道的水解,说明大鼠肠黏膜上的LPH酶参与了淫羊藿苷、朝藿定A,B,C在肠道的代谢。
Objective To study the effects of intestinal enzymes of intestine on the intestine disposal of icariin, epimedin A, epimedin B and epimedin C in Epimedium. Methods Rat intestinal perfused rat model was established. The drug concentration was determined by high performance liquid chromatography (HPLC). The effects of Epimedium saponin glycoside on the expression of gluconolactone, an inhibitor of intestinal enzymes of LPH, Changes in apparent permeability before and after. Results After adding inhibitors, the apparent permeation coefficients of four Epimedium flavonoids glycosides in the duodenum, jejunum, ileum and colon of rats all decreased to different extents, among which icariin was hydrolyzed in four intestines The inhibition rates were 62%, 50%, 40% and 46% respectively. Epimedin A was 55%, 26%, 21% and 14% respectively. Epimedin B was 42%, 40% and 74% %, 22%; Epimedin C respectively: 42%, 40%, 52%, 35%. Conclusion LPH enzyme inhibitor gluconolactone could inhibit the hydrolysis of icariin, epimedin A, epimedin B and epimedin C in rat intestine to some extent, indicating that the LPH Enzymes involved in icariin, epimedin A, B, C in the intestinal metabolism.