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采用12，25，35mg／kgbw三个剂量环磷酰胺（cyclophosphamidum,CP），经口灌胃给药，连续18天，测定小鼠的免疫器官的脏体系数，外周血T淋巴细胞ANAE阳性率，以及碳粒廓清实验的廓清指数。结果表明：CP在10mg/kgbw剂量时，除对ANAE阳性率有明显降低外，对其余两个指标的影响均不明显（P＞0.05），而在25mg/kgbw，35mg/kgbw时，对所测试的三个指标均有明显降低（P＜0．05）。说明25mg/kgbw，35mg／kgbw可以作为免疫剂量用在免疫毒理实验中，但35mg／kgbw组在给药15天后，小鼠状态不佳，有动物死亡，外周血中，红细胞明显受损，淋巴细胞减少，为ANAE阅片带来困难。所以，25mg/kgbwCP组应为免疫毒性实验中的最佳免疫剂量。
The dose of 12, 25 and 35 mg / kg bw cyclophosphamidum (CP) were given orally for 18 consecutive days. The numbers of dirty organs, the positive rate of ANAE in peripheral blood T lymphocytes , As well as the carbon clearance test clearance index. The results showed that: CP at 10mg / kgbw dose, in addition to a significant reduction in the positive rate of ANAE, the other two indicators had no significant effect (P> 0.05), while at 25mg / kgbw, 35mg / kgbw, All the three indicators of the test were significantly lower (P <0.05). The results showed that 25mg / kg bw and 35mg / kg bw could be used as immunological dose in immunotoxicity test. However, the mice in the 35mg / kg bw group had poor state after 15days of administration, animals died, the erythrocytes were obviously damaged in peripheral blood, Lymphopenia, ANAE reading difficulties. Therefore, 25mg / kgbwCP group should be the best immune dose in immunotoxicity experiments.