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目的探讨苍耳子药物血清对人脑神经胶质瘤细胞(H4细胞)的毒性作用。方法将日本大耳白兔分别于给药前灌胃生理盐水5ml/kg(给药前对照组)及灌胃苍耳子药物血清3.6g/kg(低浓度组)、7.3g/kg(中浓度组)和15.0g/kg(高浓度组),2h后从心脏抽血2ml/kg,离心取血清冻存。对H4细胞给予给药前对照组血清、不同浓度苍耳子药物血清及5-氟尿嘧啶(阳性对照组)培养48h后,用MTT法根据光密度值(OD)计算其对H4细胞的生长抑制率,用流式细胞仪检测其对H4细胞凋亡的影响。结果对H4细胞分别给予低、中、高浓度苍耳子药物血清和5-氟尿嘧啶,细胞生长抑制率分别为43.21%、49.38%、69.13%和61.72%;其各OD值与给药前对照组OD值相比,差异均有统计学意义(P均<0.01)。流式细胞仪检测发现,在对H4细胞给予低、中、高浓度苍耳子药物血清后,H4细胞的凋亡率随给药浓度的增加而升高,分别为15.1%、22.6%和25.4%。结论苍耳子药物血清对H4细胞分裂增殖具有明显的毒性和抑制作用。
Objective To investigate the toxic effects of Xanthium sibiricum sera on human glioma cells (H4 cells). Methods Japanese white rabbits were intragastrically administered with saline 5ml / kg (control group before administration) and 3.6g / kg (low concentration group) Concentration group) and 15.0g / kg (high concentration group). After 2h, blood was drawn from the heart at 2ml / kg and centrifuged to secrete serum. The H4 cells were pretreated with control serum, different concentrations of Xanthium drug serum and 5-Fluorouracil (positive control group) for 48h. The growth inhibition rate of H4 cells was calculated by MTT method according to the optical density (OD) Flow cytometry was used to detect the effect on apoptosis of H4 cells. Results H4 cells were given low, medium and high concentrations of Xanthium serum and 5-fluorouracil, respectively. The cell growth inhibition rates were 43.21%, 49.38%, 69.13% and 61.72%, respectively. OD value, the differences were statistically significant (P all <0.01). The results of flow cytometry showed that the apoptosis rate of H4 cells increased with the increase of the drug concentration, which were 15.1%, 22.6% and 25.4%, respectively %. Conclusion Xanthium drug serum on H4 cell division and proliferation has obvious toxicity and inhibition.