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目的 调查广州市交通警察人群谷胱苷肽硫酶GSTM1(GlutathioneS -transferasesM1)基因多态性分布。方法 采用人群普查和多重差异PCR(multi-differentialPolymerasechainreaction ,MD -PCR)方法对广州市交通警察外周血白细胞DNA的GSTM1基因缺失型进行检测 ,比较GSTM1基因在性别、籍贯、民族、家族肿瘤史、工作性质、工龄及每周接触汽车废气时间等方面的分布 ,并与其它地区人群进行比较。结果 广州市 2 0 93名在岗交通警察GSTM 1基因缺失率为 5 9 5 8% ,高于上海市区人群 4 8 86 % (P <0 0 5 ) ,低于云南籍肺癌人群 76 79% (P <0 0 5 )。性别、籍贯、民族、肿瘤家族史、工作性质、工龄每周接触汽车废气时间等方面没有显著性差异 (P >0 0 5 )。结论 研究人群GSTM1基因呈多态性分布 ,其等位基因和基因型率在研究人群内部分布无显著性差异。
Objective To investigate the distribution of glutathione S-transferase (GSTM1) gene in Guangzhou traffic police. Methods GSTM1 gene deletion in peripheral blood leucocyte DNA of Guangzhou traffic policemen was detected by census and multi-differential polymerase chain reaction (MD-PCR). The differences of GSTM1 gene in gender, origin, ethnicity and family history of cancer were analyzed. Nature, length of service and weekly exposure to vehicle exhaust time distribution, and compared with other regions of the population. Results The deletion rate of GSTM1 gene in 2,093 traffic police officers in Guangzhou was 59 58% higher than 486% in Shanghai (P <0 05), lower than 76 79% in Yunnan P <0 0 5). There was no significant difference in sex, origin, ethnicity, family history of the cancer, nature of work, weekly exposure to vehicle exhaust, etc. (P> 0.05). Conclusions There was no significant difference in the distribution of GSTM1 gene between the study population and the alleles and genotypes.