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目的观察七氟烷预处理对糖尿病大鼠肾脏缺血再灌注(I/R)损伤肾功能及肾组织Src和FAK表达的影响。方法将糖尿病大鼠分为假手术组、I/R组和七氟烷预处理组。给药后喂养24 h,麻醉大鼠并下腔静脉采血,测定血肌酐(creatinine,Cr)和尿素氮(blood urea nitrogen,BUN),取肾组织使用免疫组织化学方法测定大鼠肾脏组织中Src和FAK的表达。结果与假手术组比较,I/R组Cr、BUN明显增高,肾组织Src、FAK表达显著降低(P<0.05);与I/R组比较,七氟烷预处理组血清Cr、BUN明显降低,肾组织Src、FAK表达显著升高(P<0.05)。结论七氟烷预处理能改善糖尿病大鼠肾脏I/R损伤肾功能,上调Src、FAK在肾组织中的表达。
Objective To observe the effect of sevoflurane preconditioning on renal function and renal expression of Src and FAK in kidneys with ischemia / reperfusion (I / R) injury in diabetic rats. Methods Diabetic rats were divided into sham operation group, I / R group and sevoflurane preconditioning group. After administration for 24 h, rats were anesthetized and the inferior vena cava was collected for blood sampling. Creatinine (Cr) and blood urea nitrogen (BUN) were measured. The renal tissues were harvested for immunohistochemistry for the determination of Src And FAK expression. Results Compared with the sham operation group, Cr and BUN in I / R group were significantly increased, and the expression of Src and FAK in renal tissue was significantly decreased (P <0.05). Compared with I / R group, serum Cr and BUN in sevoflurane preconditioning group were significantly decreased , And the expression of Src and FAK in renal tissue were significantly increased (P <0.05). CONCLUSION Sevoflurane preconditioning can improve the kidney function of diabetic rats with I / R injury and up-regulate the expression of Src and FAK in renal tissues.