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本文就中草药抗结核杆菌试验中的实验条件,包括接种菌量、培养基的最适 pH、甘油含量、氮源原料以及中草药提取物常用溶剂——二甲基亚砜、乙醇的含量对结核杆菌生长影响等问题进行探讨。在研究中草药抗结核杆菌的试验中,首先需作试管内的抑菌试验。为了排除有机成份对抑菌作用的影响,大多数学者均采用综合培养基。但由于结核杆菌生长缓慢、管养条件要求特殊,在简单的综合培养基上,若生长条件控制不好,生长不旺盛,
In this paper, the Chinese herbal medicine anti-tubercle bacilli experimental conditions, including inoculation bacteria, the optimum pH of the medium, glycerol content, the nitrogen source of raw materials and herbs commonly used solvents - dimethyl sulfoxide, ethanol content of Mycobacterium tuberculosis Growth and other issues to be explored. In the study of Chinese herbal medicine against Mycobacterium tuberculosis, the first test tube for antibacterial test. In order to exclude the impact of organic ingredients on the bacteriostasis, most scholars use synthetic medium. However, due to the slow growth of Mycobacterium tuberculosis, nutrient conditions require special, in a simple comprehensive medium, if the growth conditions of poor control, growth is not strong,