,Protein Product Encoded by a Human Novel Gene E9730 Enhances AP-1 Activity through Interacting with

• 作 者: Zhao-Qing WANG Han-Dong WEI Fu-Chu HE
• 机 构: Department of System Biology, Beijing Institute of Radiation Medicine, Chinese Human National Center
• 出 处: 生物化学与生物物理学报
• 发表日期: 2004年1期
• 关键词: human E9730 gene CSN5/Jab1 protein-protein interaction signaling transduction

A novel human gene, named E9730 (a clone number of fetal liver cDNA library), has been identified from more than 14,000 expressed sequence tags (ESTs) based on our large scale sequencing of human fetal liver cDNA libraries. Although sequencing of this novel human gene indicates that it is a leucine zipper protein, the function of E9730 and its homologous genes among species is unknown yet. To find out physiological functional clue of E9730, the yeast two-hybrid system was used to screen the E9730-interacting protein(s), and one clone containing a cDNA insert with almost the entire coding sequence (amino acids 39-335) of human Jab1 (Jun-activating domain binding protein 1) that interacted specifically with E9730 was identified. A specific association between Jab1 and E9730 was shown by co-immunoprecipitation and co-localization experiments. Furthermore, E9730 appeared to enhance Jab1-induced AP-1 activity in a concentration-dependent manner and Jab1 may be involved in the intracellular signaling transduction from E9730 to AP-1.