,A Lectin from Chinese Mistletoe Increases γδ T Cell-mediated Cytotoxicity through Induction of Casp

• 作 者: Fang GONG Yanhui MA Anlun MA Qiwen YU Jiying ZHANG Hong NIE Xuehua CHEN Baihua SHEN Ningli LI Dongqing ZHANG
• 机 构: Shanghai Jiaotong University School of Medicine, Shanghai Institute of Immunology, Shanghai 200025,
• 出 处: 生物化学与生物物理学报（英文版）
• 发表日期: 2007年6期
• 关键词: mistletoe lectin γδ T cell cytotoxicity apoptosis cytokine

In this study, a mistletoe lectin (ML) was purified from Chinese mistletoe and the effect of this 60 kDa Chinese ML on human γδ T cell cytotoxicity, apoptosis and modulation of the cytokine network was studied. The cytotoxic properties of δ T cells was evaluated by using a 51Cr release test and employed fluorescence-activated cell sorting and enzyme-linked immunosorbent assay analysis to quantify translocation of the cell membrane phospholipid, phosphatidylserine and nuclear DNA fragmentation during apoptosis.It was found that: (i) ML effectively stimulated γδ T cell proliferation in a dose- and time-dependent manner;(ii) ML increased γδ T cell cytotoxicity; (iii) ML could modulate lipopolysaccharide-induced cytokine release in a pro-inflammatory manner by increasing tumor necrosis factor (TNF)-α release and inhibiting the release of anti-inflammatory interleukin (IL)-10; (iv) ML induced apoptosis in caspase-dependent and CD95-independent manner. The results indicated that ML is a potent immunomodulator to human γδ T cell cytotoxicity, apoptosis and cytokine production.