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利用免疫荧光显微镜技术和免疫胶体金标记技术确定蚕豆气孔保卫细胞中存在一氧化氮合酶(NOS)类似蛋白,NOS主要分布在气孔保卫细胞的细胞核、细胞质、叶绿体、线粒体以及细胞壁上.局部灼伤和外源茉莉酸(JA)都能提高蚕豆叶片和表皮NOS活性和一氧化氮(NO)水平,NOS的活性变化与叶片中的NO的变化趋势基本一致;NOS抑制剂L-NAME可抑制JA诱导的NO水平的增加.由此推测,NOS途径是伤胁迫和JA诱导形成NO的主要途径.药理学实验表明适当增加Ca2+浓度能够提高叶片NOS的活性和NO的水平,说明蚕豆叶片NOS活性和NO的分布具有一定的钙依赖性.保卫细胞中NOS及其催化形成的NO可能通过对气孔运动的调节参与植物对逆境的响应.
Immunofluorescence microscopy and immunogold labeling techniques were used to determine the presence of nitric oxide synthase (NOS) -like proteins in Vicia faba guard cells. NOS mainly distributed in the nuclei, cytoplasm, chloroplast, mitochondria and cell walls of stomatal guard cells. And exogenous jasmonic acid (JA) increased NOS activity and nitric oxide (NO) levels in broad bean leaves and epidermis, respectively. The change of NOS activity was basically consistent with that of leaf NO. NOS inhibitor L-NAME inhibited JA Suggesting that NOS pathway is the main pathway of injury-induced stress and JA-induced NO formation.Physiological experiments show that Ca2 + concentration can increase NOS activity and NO level in leaves, indicating NOS activity and The distribution of nitric oxide (NO) has a certain calcium dependency.The NOS in guard cells and its catalyzed formation of NO may be involved in plant response to stress through the regulation of stomatal movement.