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三维电纺纤维在生物医学领域,如生物传感、药物控制释放与组织工程等方面具有良好的应用前景.然而,现有的电纺技术在制备结构、孔隙率与形貌均可调节的三维定向电纺纤维方面还存在一定不足.因此亟需开发一种新型的电纺丝工艺以制备三维定向电纺纤维.本文通过改进传统的电纺丝工艺,开发了一种简单高效制备三维定向聚偏氟乙烯(PVDF)的电纺丝制备技术.所制备的三维定向纤维的形貌、直径及纤维密度均可控.体外细胞实验结果表明,该类三维定向纤维具有良好的生物相容性,能够促进细胞活性,诱导细胞沿着纤维的方向生长.此外,研究结果还表明,将该三维定向纤维作为细胞培养支架时,细胞的增殖高于利用传统的二维纤维膜.该制备技术将极大地拓宽三维定向纤维在三维细胞培养、组织工程及疾病诊断等生物医学领域的应用.
Three-dimensional electrospun fibers have good application prospects in the biomedical fields such as biosensing, drug controlled release and tissue engineering, etc. However, the existing electrospinning technology has many advantages in the preparation of the three-dimensional There is still some shortages in the direction of the electrospun fibers.Therefore, it is urgent to develop a new type of electrospinning process to prepare three-dimensional oriented electrospun fibers.Through the improvement of the traditional electrospinning process, this paper developed a simple and efficient preparation of three-dimensional directional poly Vinylidene fluoride (PVDF) electrospinning preparation technology.The prepared three-dimensional oriented fibers morphology, diameter and fiber density are controlled in vitro experiments show that the three-dimensional fibers have good biocompatibility, Can promote cell activity and induce cells to grow in the direction of the fiber.In addition, the results also show that when using the three-dimensional oriented fibers as a cell culture scaffold, the cell proliferation is higher than that of the traditional two-dimensional fiber membrane, The earth widens the application of three-dimensional directional fibers in biomedical fields such as three-dimensional cell culture, tissue engineering and disease diagnosis.