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在多年的小麦(Triticum aestivum L.)花药培养育种及对多种培养基应用研究的基础上,从调整培养基的大量元素和微量元素入手,建立了癸培养基。经过L_(16)(4~5)正交实验,筛选出NH_4N0_3、KNQ_3、MgSO_4、MnSO_4在培养基中较优的配比。在培养基对比实验中,癸培养基的愈伤组织平均诱导率分别比C_(17)、W_(14)、N_6培养基提高了30.31％、50.60％和57.96％,且对不同的杂交组合都能表现出优越性。在癸培养基中附加0.9mg/L REA(rare eaxth addition,稀土元素附加剂),可使小麦花药愈伤组织诱导率提高54.25％～64.07％,并对愈伤组织的生长有促进作用。
Based on many years of breeding and breeding of anther of wheat (Triticum aestivum L.) and its application to many kinds of culture media, the decolorization medium was established by adjusting the large amount of elements and trace elements in the culture medium. Through the orthogonal experiment of L_ (16) (4 ~ 5), the optimal ratios of NH_4N0_3, KNQ_3, MgSO_4 and MnSO_4 in the medium were screened out. In medium contrast experiments, the average callus induction rate of callus culture medium was 30.31%, 50.60% and 57.96% higher than that of C_ (17), W_ (14) and N_6 medium, respectively, Can show superiority. The addition of 0.9 mg / L REA (rare eaxth addition) to the dextrose medium can increase the callus induction rate of wheat anthers by 54.25% -64.07% and promote the growth of callus.