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目的:观察雌、孕激素对人绒癌细胞系JEG-3腺苷受体表达的影响,探讨它对JEG-3侵袭性的影响。方法:JEG-3细胞在含雌、孕激素环境中培养24h,设MRS1706(A2BR阻断剂)干预组及空白对照组;用明胶酶谱法检测MMP-2、MMP-9蛋白表达,RT-PCR技术检测JEG-3细胞腺苷受体mRNA水平。结果:RT-PCR结果显示,JEG-3细胞表达腺苷A1R、A2AR及A2BR3个亚型,未检测到A3R的表达;雌激素和(或)孕激素处理组A2BRmRNA含量显著高于对照组(P<0.05);明胶酶谱检测发现雌、孕激素联合处理显著上调JEG-3细胞MMP-2的分泌水平(P<0.05),但对MMP-9无显著影响;阻断A2BR信号途径显著下调JEG-3MMP-2表达,并能抑制雌、孕激素对MMP-2的上调效应(P<0.05)。结论:雌孕激素能改变JEG-3绒癌细胞腺苷受体的表达模式,呈A2BR优势表达,A2BR信号通路参与雌孕激素对JEG-3细胞侵袭性的调节。
Objective: To observe the effect of estrogen and progesterone on the expression of adenosine receptor 3 (JEG-3) in human choriocarcinoma cell line and to investigate its effect on the invasiveness of JEG-3. Methods: The JEG-3 cells were cultured for 24 h in estrogen-progesterone-containing medium. MRS1706 (A2BR blocker) intervention group and blank control group were established. Gelatin zymography was used to detect the expression of MMP-2 and MMP- PCR technique was used to detect the adenosine receptor mRNA level in JEG-3 cells. Results: RT-PCR results showed that A3R was not detected in JEG-3 cells expressing adenosine A1R, A2AR and A2BR. The content of A2BR mRNA in estrogen and / or progestin group was significantly higher than that in control group <0.05). Gelatin zymography showed that combined treatment of estrogen and progesterone significantly upregulated the secretion of MMP-2 in JEG-3 cells (P <0.05), but had no significant effect on MMP-9; blockade of A2BR signaling pathway down-regulated JEG -3MPMP-2 expression, and can inhibit the estrogen and progesterone on MMP-2 up-regulation effect (P <0.05). CONCLUSION: Estrogen and progesterone can change the expression pattern of adenosine receptor in JEG-3 choriocarcinoma cells and express A2BR predominantly. The A2BR signaling pathway is involved in the regulation of estrogen and progesterone on invasiveness of JEG-3 cells.