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目的探讨Parasep~?粪便离心管法在血吸虫病病原学检测中的应用效果。方法选择湖北省江陵县2个血吸虫病流行村6~65岁居民为研究对象,采用改良加藤厚涂片法(Kato-Katz法)、尼龙绢集卵孵化法和Parasep~?粪便离心管法进行病原学平行检测,对检测结果进行比较分析。结果 2个村共检测803人,共查出血吸虫虫卵阳性15例,平均粪检阳性率为1.87%。其中Kato-Katz法、尼龙绢集卵孵化法和Parasep~?粪便离心管法粪检阳性率分别为0.75%、1.49%和1.12%,Parasep~?粪便离心管法的检出率是Kato-Katz法的1.49倍。2个村Kato-Katz法的漏检率最高,分别为57.14%和62.50%,尼龙绢集卵孵化法最低,分别为14.29%和25.00%。Parasep~?粪便离心管法可使显微镜下虫卵形态不变,卵内毛蚴结构清晰,容易辨认。结论在血吸虫病低度流行区,Parasep~?粪便离心管法可作为日本血吸虫病病原学诊断的方法。
Objective To investigate the application of Parasep ~? Feces centrifuge tube method in the etiological detection of schistosomiasis. Methods Residents of 6 to 65 years old in 2 schistosomiasis endemic villages in Jiangling County of Hubei Province were selected as research objects. Kato-Katz method, nylon egg hatching method and Parasep ~ Etiology parallel test, the test results were compared. Results A total of 803 people were detected in 2 villages. A total of 15 cases of schistosomiasis eggs were detected, and the average positive rate of stool examination was 1.87%. The positive rates of Kato-Katz method, nylon silk hatching method and Parasep ~? Fecal centrifugation method were 0.75%, 1.49% and 1.12% respectively. The detection rate of Parasep ~? Faecal centrifugation method was Kato-Katz 1.49 times of the law. The Kato-Katz method in two villages had the highest detection rate of 57.14% and 62.50%, respectively. The lowest egg hatching rate was 14.29% and 25.00% respectively. Parasep ~? Faecal centrifuge tube microscope morphology can change the egg, miracidia eggs clear structure, easy to identify. Conclusions In the endemic areas of schistosomiasis, the Parasep ~? Faecal tube method can be used as a method for the etiological diagnosis of schistosomiasis japonica.