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用抗甲胎蛋白（ａｎｔｉ－ＡＦＰ）的单克隆抗体亲和层析柱从脐带血中提纯ＡＦＰ。通过ＭＴＴ法体外观察人ＡＦＰ对Ｈ－２２腹水肝癌小鼠脾淋巴细胞增殖的影响。结果表明，Ｈ－２２小鼠脾淋巴细胞经不同浓度ＡＦＰ（１２．５－１００μｇ／ｍｌ）处理后，对刀豆素Ａ（ＣｏｎＡ）诱导的增殖反应明显减弱，抑制率达３９．８％－６６％，并有一定的浓度依赖关系；而用不同浓度的人血清白蛋白（ＨＳＡ，３．１～１００μｇ／ｍｌ）体外处理，则无明显抑制作用。另外，我们用反证法证实，ＡＦＰ抗体（５５、１１０μｇ／ｍｌ）能明显阻断人ＡＦＰ（５０、１００μｇ／ｍｌ）对Ｈ－２２小鼠脾淋巴细胞增殖的抑制作用。
AFP was purified from cord blood using an anti-AFP monoclonal antibody affinity column. The effect of human AFP on the proliferation of spleen lymphocytes of H-22 ascitic hepatocellular carcinoma mice was observed in vitro by MTT assay. The results showed that after treatment with different concentrations of AFP (12.5-100 μg/ml), the spleen lymphocytes of H-22 mice were significantly attenuated against ConA-induced proliferative responses, with an inhibition rate of 39.8%- 66%, and there is a certain concentration dependence; and with different concentrations of human serum albumin (HSA, 3.1 ~ 100μg/ml) in vitro treatment, there is no significant inhibitory effect. In addition, we used reverse-proved methods to confirm that AFP antibody (55,110 μg/ml) can significantly block the inhibitory effect of human AFP (50,100 μg/ml) on spleen lymphocyte proliferation in H-22 mice.