Identification of candidate piRNAs in the gonads of Paralichthys olivaceus (Japanese flounder)

• 作 者: Chun-Lei WANG Zhi-Peng WANG Jia-Qi WANG Ming-You Li Xiao-Wu CHEN
• 机 构: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, S
• 出 处: Zoological Research
• 发表日期: 2016年05期
• 关键词: Paralichthys olivaceus Ovary Testis piRNA

Piwi-interacting RNA(piR NA) plays an important role in the gonadal development and maintenance of Teleostei. In this study, pi RNA libraries derived from the adult gonads of Japanese flounder(Paralichthys olivaceus) were generated using next-generation sequencing technology. Using zebrafish piR NAs as a reference, 5 865 unique candidate pi RNAs were identified; 289 candidate piR NA clusters(PRCs) were generated from the above pi RNAs. Among the isolated candidate PRCs, a total of 38 ovary-specific, 45 ovary-bias, 24 testis-specific, and 131 testis-bias PRCs were found. The relative expression levels of seven PRCs were validated through quantitative reverse transcription-polymerase chain reaction. The results of this study will help facilitate exploration of the development and maintenance of the phenotypic sex mechanism in P. olivaceus.

Pi this work, pi RNA libraries derived from the adult gonads of Japanese flounder (Paralichthys olivaceus) were generated using next-generation sequencing technology. Using 289 candidate piR NA clusters (PRCs) were generated from the above pi RNAs. Among the isolated candidate PRCs, a total of 38 ovary-specific, 45 ovary-bias , 24 testis-specific, and 131 testis-bias PRCs were found. The relative expression levels of seven PRCs were validated through quantitative reverse transcription-polymerase chain reaction. The results of this study will help facilitate exploration of the development and maintenance of the phenotypic sex mechanism in P. olivaceus.